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H3K9me3 and H3K27me3 histone marks promote heterochromatin formation, reinforcing senescence-associated gene silencing.21 Senescent cells also exhibit DNA methylation changes, altering gene expression patterns.22 Senescence in Aging and Age-Related Diseases The role of cellular senescence in aging is multifaceted

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TGM assay The assay was performed in polylysine-coated microtiter wells