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Immunohistochemistry For immunohistochemistry, paraffin sections of liver and intestine specimens were deparaffinized, rehydrated in decreasing concentrations of ethanol, treated for 10 minutes with 3% H 2 O 2 to quench endogenous peroxidase activity and heated to 100C for 1 h in 10 mM sodium citrate buffer, pH 6, for antigen retrieval

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However, protein levels of CII and CIV were also affected, thus implying that other modifications (i.e

Schairer DO, Chouake JS, Kutner AJ, Makdisi J, Nosanchuk JD, Friedman AJ

2A) and inhibited VEGFA and IL8 induction by erastin at the mRNA, intracellular, and secretory protein levels (Fig