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Description
IM (Intramuscular)Twice per week, in your upper thigh muscle SC (Subcutaneous) Up to 5 times per week in your belly 2 inches from your belly button

Now that's the media side
You push the needle past the subcutaneous layer and into the joint capsule

The postload sampling test does burden biomedical protocols, however, this test was shortened to 2 hours in a recent study (Bostom et al, 1995b)

The cultures were shaken at 20 C and 240 rpm for 65 hours
