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To exclude artifactual alterations by the FITC group, we performed kinetic-binding experiments with unlabeled agonists, in competition with exendin-4-FITC, and defined both association rate constants and residence times 43 with consistent effects (Fig

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In addition, glutamate and alanine in positions 21 and 24 respectively were reversed, and a glycine residue at position 29 was replaced by asparagine