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The homogenate was filtered with a 40-m cell strainer and centrifuged at 600 g for 3 min at 4 C
elegans , lipid-specific staining methods, such as Nile Red or Oil Red O (ORO) staining, are typically used in combination with chromatography-based mass spectrometry (MS) 17,18
The lesions may be flat or slightly raised and can grow and spread rapidly
Samples were prepared by mixing volumes of desired protein and ligand such that the final protein concentration in each case is 5 M, C 55 -P at 20 M, lipid I at 5 M, lipid II at 5 M
PMID: 32108443
