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2015 NNMT consumes SAM as a methyl donor, competing with DNA and histone methyltransferases
Think of it like this: a doctor needs an X-ray to confirm a broken bone before putting on a cast

However, individuals who are vulnerable to high doses of B12 in practice are few and far between

36 38 39 However, MMA was normal in 52% of individuals with holoTC concentrations below 20 pmol/L, the latter being indicative of deficiency

Lyophilization is a fancy term for freeze-drying, a process that removes water and makes the peptide stable for shipping and storage
