ellman 1959 reduced glutathione Assay (Colorimetric) The Use of Different MS

Owned and controlled by individuals who are U.S

The C-terminal 6xHis-tagged LbcA protein was purified with HiTrap-Ni in 10 mM potassium phosphate, pH 8.0, 0.25 M NaCl, and a 20-CV 10300 mM imidazole gradient, followed by HiTrap-Q in 10 mM Tris, pH 8.0, and a 20-CV 50500 mM NaCl gradient

The study was funded by the Max Planck Society, the German Registry of COVID19 Autopsies (www.DeRegCOVID.ukaachen.de, ZMVI12520COR201) and the Bavarian Ministry of Science and the Arts

96 well plates were coated with capture antibody overnight at room temperature and then washed three times in PBS plus 0.1% Triton X-100 (PBST)

[DOI] [PMC free article] [PubMed] [Google Scholar] 3.Khan M., Ali S., Al Azzawi T.N.I., Saqib S., Ullah F., Ayaz A., Zaman W