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Impact of the gut microbiota on inflammation, obesity, and metabolic disease

Total protein extracts were obtained by homogenization of tissues using protein sample buffer [100 mM Tris-HCl (pH 6.8), 200 mM dithiothreitol, 4% sodium dodecyl sulfate (SDS), 0.2% bromphenol blue and 20% glycerol] and a classic protease inhibitor cocktail (Beyotime Institute of Biotechnology)

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Figure 1 L-carnitine, a TMA found abundantly in red meat, has aroused considerable research interest because an increasing number of studies have found that it is associated with atherosclerosis ( via intestinal flora-derived CntA/CntB , and also into -butyrobetaine and betaine, and further converted to TMA through the yeaW/yeaX enzyme system of intestinal flora (Figure 1

Cryo-EM data processing All image processing and map reconstruction was performed using Cryosparc v 4.6.0