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Additionally, it reversibly acetylates the K1371 site on TSC2, boosting how well its GAP domain interacts with Rheb.Isotope tracing experiments have confirmed that in GCN5L knockout cells grown in an amino acid-free medium, the activity of glutamine synthetase (GLUL) decreases, while the production of -ketoglutarate dependent on glutamate dehydrogenase (GLUD1) is reduced, resulting in the disruption of the tricarboxylic acid cycle metabolic flow (Miao, 2023)

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