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For human fibroblasts, cells were fixed with methanol/acetone (1:1) for 15 min, permeabilized in 0.1% (v/v) Triton X-100 for 15 min and incubated with primary antibodies overnight at 4 C

However, it is necessary to verify their clinical benefits and toxicity risks

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These results chiefly represent correlative analyses between tumor gene expression and existing datasets

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