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Control and treated cells were collected and lysed in ice-cold RIPA buffer (phosphate buffer saline pH 7.4 containing 0.5% sodium deoxycholate, 1% Igepal P-40, 0.1% SDS, 5 mM EDTA, 1% protease, and phosphatase inhibitor cocktails, Sigma-Aldrich, USA)

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PMID 24791752 Weschawalit S, et al

Dystonia in Wilsons disease