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After UDG enzyme (NEB, cat.m0280, USA) treatment of U-labeled second-strand DNA, the ligated products were PCR-amplified under the following conditions: initial denaturation at 95 C for 3 min
As cumulus cells possess their own oxidative defence capacity 66, 67 , we elected to denude oocytes prior to treatment
doi: 10.1515/CCLM.2008.349
doi: 10.1016/j.neuropharm.2018.07.014
