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Description
CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

A report from the American Society of Plastic Surgeons adds another perspective

Islam A, Mishra A, Ahsan R, Fareha S

CCI, chronic constriction injury

This biphasic dose-response demonstrates that "more is not better" there is an optimal concentration window for copper peptide efficacy
