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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)

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Muscle Regeneration Research Models Standard rodent muscle protocols include crushed gastrocnemius, transected quadriceps, and myotendinous junction dissection models