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Material and methods Genomic DNA samples were extracted from 297 healthy unrelated individuals by a ReliaPrep Blood gDNA Miniprep kit (Promega, USA), and genotyped for allelic variation in GSTM1 and GSTT1 genotypes by multiplex polymerase chain reaction

Hereditary Hemochromatosis (HH), a common genetic disorder causing iron overload, significantly elevates the risk of cancer, particularly hepatocellular carcinoma (liver cancer), with some studies reporting a 200-fold increased incidence.