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Englewood, NJ: Biostat 104, (2005)

Intestinal microbiota-generated metabolite trimethylamine-N-oxide and 5-year mortality risk in stable coronary artery disease: the contributory role of intestinal microbiota in a COURAGE-like patient cohort

Resulting intact mitochondria were resuspended in Elution Buffer containing 250 mM sucrose, 0.02 mM EGTA, 10 mM Tris pH 7.4, 2 mM Tris-phosphate pH 7.4, and 5 mM MgCl 2