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Phosphate starvation in fungi induces the replacement of phosphatidylcholine with the phosphorus-free betaine lipid diacylglyceryl-N, N N-Trimethylhomoserine

Time-lapse images were acquired every 20 min for 20 h using the epifluorescence microscopy mode

Does the procedure hurt

however, as Wood (1987:115) notes, fingernail indentations are occasionally seen on prehistoric plainwares across central Arizona as well