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Positive serum samples were purified using a protein A column and eluted with 150 mM glycine buffer

Lambrechts, M J., Schroeder, G.D., Tran, K., Li, S., Huang, A., Chu, J., Karamian, B.A., Canseco, J.A., Hilibrand, A.S., Oner, C., Dvorak, M., Schnake, K., Kepler, C.K., Vaccaro, A.R.: Validation of the AO Spine Thoracolumbar Injury Classification System Treatment Algorithm: Should it be used to Guide Fracture Management

This ensures the maintenance of whole-body homeostasis that is essential for life in animals including fish

Cells were harvested 72 h following seeding and compounds were added 1, 4 or 24 h before cell harvest

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