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miyamotoi a relapsing fever species found in the same blacklegged ticks that carry Lyme)

AT, alpha-1-antitrypsin (A1AT) and FVII were evaluated by western blots on 8% polyacrylamide gel electrophoresis performed under 10% SDS denaturing conditions in the presence 0.05 M reducing agent

They used spectrofluorimetric method with orthophthalic aldehyde (OPT), UV/visible spectroscopy using DTNB, and HPLC