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Where literature is mixed or evidence is preliminary, the article hedges rather than overclaims

Analytical conditions for optical purity: Daicel ADH column (0.46 25 cm), n-hexane (added with one thousandth of diethylamine)/ethanol = 80/20, 0.5 mL/min, = 254 nm, tR = 40.66 (R), tS = 64.22 (S)

This enzyme catalyzes the N-methylation of nicotinamide using S-adenosyl-L-methionine (SAM) as a methyl donor, effectively creating a "metabolic drain" that depletes both NAD+ precursors and cellular methylation capacity

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