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For example, NMR-titration experiments of non-glutathionylated poplar GrxC4 or ScGrx8 with GSH required high glutathione concentrations to detect chemical shifts 47,48 (and titrations of active site serine mutants with GSSG cannot discriminate between both glutathionyl moieties)

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The MAMs serve several functions, one of which being that the SR and mitochondria can transfer Ca 2+ at MAMs [Figure 4 (49)]

5 Oxidative stress Elevated levels of free radicals, encompassing both reactive nitrogen species (RNS) and reactive oxygen species (ROS), may arise due to secondary cell death and oxidative stress

Cell Culture and GSH Preparation K562, HepG2, Hela and L-02 cells were respectively cultured in RPMI 1640 (Hyclone, penicillin 100 U/mL, streptomycin 100 g/mL) adding 10% fetal bovine serum and maintained at 37 C in a humidified atmosphere including 5% CO 2 , in terms of the instructions of the American Type Culture Collection