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PC12-ND6 cells were seeded at a density of 20,000 cells per well in triplicate on poly-d-lysine-coated plates and treated with PBS (control) or NaBt (5 mM) for 24 h at 37 C in 5% CO2 atmosphere
For vir-1-GFP signal detection in brain, we used an LSM800 (Zeiss) inverted laser scanning confocal microscope
