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Instead, they recommended methylcobalamin, a form of B12 where a methyl group replaces the cyano group

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FEBS J 274:21632180 Hicks LM, Cahoon RE, Bonner ER, Rivard RS, Sheffield J, Jez JM (2007) Thiol-based regulation of redox-active glutamate-cysteine ligase from Arabidopsis thaliana

Tris-BSAN: prepare 50 mM Tris buffer in water with serine/boric acid/acivicin/NEM (pH 8.0)