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Figure 2. Retained His-tagged protein binding capacity in presence of EDTA. Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280

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COMPOSICIN Agua, L-Carnitina 1500 mg, Acetil L-Carnitina 1500 mg, Coenzima Q10 30 mg, Aroma de limn (Aroma), Potasio sorbato (Conservante), cido ctrico (Acidificante), Sucralosa (Edulcorante OPININ FARMACETICA Los complementos alimenticios no deben utilizarse como sustitutivos de una dieta equilibrada y por un modo de vida sano

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