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However, many factors, including their low stability, short half-life, high immunogenicity, tissue targeting, cellular uptake, and endosomal escape, hamper the delivery and clinical application of NADs

Huang Y, Gan Q, Lai R, et al

H.KunchokT.et al

However, to date, oligonucleotide therapeutics are still facing some technological obstacles, such as how to achieve efficient oligonucleotide delivery to target organs or tissues, how to overcome off-target interactions [4952], and how to address the sequence- and chemistry-dependent toxicity and saturation of endogenous RNA processing pathways [53, 54]

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