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Acknowledgements All figures were created with Biorender.com

The chondral defect (4 mm in diameter) was created in the trochlear groove of the distal femur according to a previously described method [24]

Our analysis indicated that the cellular differentiation trajectory started with M-MCEMP1 and subsequently exhibited two principal differentiation branches, with M-SPP1 in the beginning, M-ISG15 and M-proliferating near the first branch point, ending with M-CXCL9 and M-CXCL2, and M-SELENOP, respectively (Fig

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