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Bifidobacterium species, obligate anaerobes colonizing the distal small intestine and colon, support gut homeostasis by metabolizing dietary substrates, inhibiting pathogens, modulating inflammatory responses, maintaining barrier integrity, and contributing to micronutrient and bioactive compound production, with emerging evidence for antitumor potential (23, 24)

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Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development