how to reverse glutathione deficiency system enhancement for cardiac protection: pharmacological options against oxidative stress and ferroptosis Potential Role of Glutathione Antioxidant

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Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)

However, since lysine is usually exposed on the antibody surface, different amounts of drug loading attach to different positions, resulting in a higher degree of heterogeneity of the conjugate

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