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For instance, oral administration might be subject to first-pass metabolism in the liver, reducing the effective concentration of 5-Amino-1MQ compared to a direct subcutaneous injection

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Tissue or cell immunofluorescent staining For immunohistochemical staining, kidney sections were immersed in xylene followed by decreasing concentrations of alcohol (100%, 95%, 90%, 85%, and 75%) to produce 5 m thick sections, and washed thrice using phosphate buffer solution (PBS)

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