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2 Methodology A comprehensive literature search was conducted in scientific databases including PubMed, Web of Science, ScienceDirect, Google Scholar, WanfangData, Chinese Pharmacopoeia, and China National Knowledge Infrastructure (CNKI) using keywords such as Astragalus , Astragalus L., Astragalus spp., Astragalus , Astragali radix , and Astragali complanati semend

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The current review describes the interrelationships between ferroptosis and energy metabolism with an emphasis on the intermediate role of iron, and the role of these processes under normal and diseased conditions

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Briefly, different concentrations of TLC-2716 or TLC-6665 were incubated with an assay buffer (Tris/HCl buffer (pH 6.8) containing 240 mM KCl, 1 g l 1 bovine serum albumin and 0.002% Triton X-100) containing 530 g ml 1 recombinant GST-tagged LXR/LXR LBD, 125 pg l 1 GST-Tb cryptate (CisBio), 400 nM N-terminally biotinylated NCOA3 (coactivator) peptide, 1 M 24( S )-25-epoxycholesterol (LXR agonist, only in the NCOA3 assay) and 2.5 ng l 1 Streptavidin-XL665 (CisBio) for 1 h at 4 C, following which fluorescence was measured in a VictorX4 multiplate reader (PerkinElmer Life Science) using 340 nm as excitation and 615 and 665 nm as emission wavelengths