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Description
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2.9.4 Estimate lactate dehydrogenase activity LDH assessment was performed in serum using a reaction mixture containing 100 L of NADH (0.02 M), 0.2 mL of serum, 0.1 mL of sodium pyruvate (0.01 M), and 1.1 mL of phosphate buffer (0.1 M) at physiological pH
Nine hours later, animals were euthanized using carbon dioxide (CO 2 ) in a controlled chamber with a volume displacement rate of 20% per minute
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