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To score -butyrobetaine hydroxylase activity, an L-carnitine biosensor strain was used, and fluorescence was measured upon extracellular addition of -BB
[DOI] [PMC free article] [PubMed] [Google Scholar] 47.Ehmke M., Luthe K., Schnabel R., Dring F
Pucci MJ Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae
requires the holin-like protein TcdE
