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The first step of the pathway is the reduction of H 2 O 2 to water by the action of APX, using ascorbate acting as the electron donor
In higher plants, two different app-roaches to detect GSNO have been reported: immunohistochemical analysis using commercial antibodies against GSNO (Barroso et al., 1 fresh weight (Airaki et al., Function of GSNO under adverse environmental conditions At present, some data shows that GSNO is an important molecule in the mechanisms of response to biotic and abiotic stress

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Indeed, MDA-MB-231 breast cancer cells and non-malignant retinal pigment epithelium cells (hTERT RPE-1) as well as human foreskin fibroblasts (HFF) also showed a significant increase in cell area and FA number upon aldolase depletion but not upon PFK or GAPDH loss, demonstrating that the differential impact of glycolytic enzymes on adhesions is conserved across cell types (Extended Data Fig

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