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Methionine deprivation can cause a global decrease in histone methylation [41,45] and models of B12 deficiency have also been shown to decrease the methylation level of proteins [46,47] (Figure 2A)

One milliliter of ice-cold RNX-PLUS reagent (CinnaGen, Iran) was added to each homogenized sample
After reviewing their protocol, the cause became clear

Notably, it has been reported that authentic SARS-CoV-2 particles contain substantial amounts of uncleaved spike protein, and that the spike protein can be transported via a Golgi-bypass pathway 15,19,48 When the spike protein is exogenously expressed in HEK293 cells using plasmid vectors under non-stress inducing conditions, the majority of cell surface-expressed spike proteins are cleaved, indicating that they have been transported via a Golgi-mediated route (Fig

[PubMed] [Google Scholar] 23.Guyton AC, Hall JE
