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doi: 10.1016/j.aquaculture.2006.07.014

2.9 In vivo ubiquitylation assay HEK293T cells were transfected with the indicated siRNAs, or transfected with the indicated plasmids for 24 h followed by treatment with 20 M MG132 for 6 h, washed with PBS, and lysed in RIPA buffer containing a protease inhibitor cocktail