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PCR was carried out using 10 mM of each primer and 1ul of genomic DNA as template in 15 l reactions containing 10X Kapa Taq buffer A, 0.2 mM dNTPs, 1.5 mM MgCl 2 , 1U Kapa Taq (Kapa biosystems)

This hypothesis states that cell death in nigral neurons is caused by an interaction between environmental conditions and genetic predisposition, resulting in mitochondrial respiratory failure and oxidative stress [3]

Its small, well-defined sequence makes it a tractable probe for dissecting endogenous regulatory peptide signalling

[DOI] [PMC free article] [PubMed] 404.Hetz, C., Zhang, K

Am J Clin Nutr.2007;86(4):1167-1173