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Sets, reps, rest periods, and tempo are all important training variables that should be taken into account
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If we increase the density of labeled proteins but continue to collect the same number of photons/fluorophore, this localization precision is increased to 0.5 nm for 1000 labeled proteins/m 2 , though this is below the predicted precision of 0.3 nm that should be theoretically possible for a shot noise limited measurement (Fig
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