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The absorbance of the supernatant was measured at 450, 532, and 600 nm using a spectrophotometer

Moreover, human -defense 1 (HBD1) is a naturally occurring cationic antimicrobial peptide in the human body with essential functions such as broad-spectrum antimicrobial activity, inflammation inhibition, differentiation modulation, anticancer properties, and immune chemotaxis [139, 140]

If side effects become unmanageable, you can step back to the previous dose and try escalating again after another four weeks

Arterburn D, Wellman R, Emiliano A, et al