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All mice were maintained at 21 C on a 14/10 light/dark cycle at at 3070% humidity and given standard mouse chow (LabDiet PicoLab Rodent Diet 20) and water ad libitum with no deviation prior to or after experimental procedures
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Site-directed, ligase-independent mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h
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