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Importantly, in all of these experiments, the supplemented groups received isonitrogenous and isocaloric solutions, i.e., both containing 13.46 g of glutamine/100 mL and 8.20 g of alanine/100 mL

After 30 min at 4 C, cells were washed in perm/wash buffer and resuspended with secondary Ab anti-Rabbit IgG FITC (Fisher Scientific 15303926) or PE (CST 79408 S)