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The only difference is how your protein of interest is connected to the agarose beads, which has important implications for how you elute it, and any proteins that it binds to from the column

Coskun T, Sloop KW, Loghin C, et al

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Abstract Sarcopenia is an age-related syndrome characterized by a progressive and extensive decline in skeletal muscle mass and function, leading to serious impairment of the quality of life and poor health prognosis in older adults