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MMP activation primarily occurs via proteolytic cleavage (by Ser proteases or other MMPs) or by modifying the thiol group by oxidation (for example, through reactive oxygen species generated by leukocytes)
Overall, current evidence indicates that men with palpable varicocele have increased sperm DNA fragmentation levels than men without varicocele
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Following blocking, membranes were incubated with primary antibodies overnight at 4C
