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For F-actin quantification, at the end of experiment, cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phallodin 1 g/mL (Sigma Aldrich) during 5 min

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Although the formate-driven reaction does not use Trx or Grx and is independent of NADPH, TrxR, and Gsr, it also might not involve reduction of a disulfide bond

The Blunt Truth About Glutathione Regulation Here's the honest answer: the FDA doesn't regulate glutathione the way most people assume federal agencies regulate medications

Oeda, T
