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Caspase 3 activity assay Fifty milligrams of frozen remnants livers were lysed in caspase 3 activity buffer (20 mM PIPES, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS, 10% sucrose, pH 7.2)

While oral supplementation can be beneficial, it may not be as effective as direct administration methods

In vitro, we confirmed that CTP did not target lung fibroblasts with or without TGF- stimulation (Supplementary Fig

Results from these multi-scale approaches reveal that cysteines 8 and 83 are the main targets of glutathionylation and oxidation that lead to loss of activity, while cysteine 79 is less vulnerable to oxidation

10.1146/annurev.pharmtox.45.120403.095857 31 HayesJ
