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Blood pH was measured using the Rapidlab 1265 (Siemens Healthcare Diagnostics BV, Den Haag, The Netherlands)

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In this study, 45 healthy adults with RLS were assigned to receive either standard management of RLS (regimen consisting of eight hours of sleep daily, 20 minutes of mild exercise four times weekly, and vitamin C plus B vitamins) or standard management plus 150 mg Pycnogenol daily for four weeks