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Although eugenol is classified as GRAS at low doses, doseresponse relationships and long-term safety profiles in MASLD conditions are not well defined, especially considering reports of hepatotoxicity at high concentrations therefore further research is required to enhance its safety profile [178]

The decrease in mitochondrial membrane potential is associated with autophagy, apoptosis, or necrosis, and is also one of the important indicators of normal mitochondrial function [28]

In terms of new research avenues in the NAD + field, it is also emerging that apart from its effects on the mitochondria, NAD + plays a multitude of roles in regulating both metabolic and non-metabolic processes

pain was assessed by VAS and pain-related quality-of-life (QoL) scores