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Immediately after washing, the cells were provided with a full cell culture medium and were placed in the incubator of the microscope (37C under a humidified 5% CO 2 atmosphere)

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479 Additional signaling pathways, including those involving PKR (EIF2AK2), PERK (EIF2AK3), lysosomal acidification, and sirtuin-regulated antioxidant programs, have also been implicated in shaping the mitochondrial stress response

By integrating metabolic regulation with inflammation suppression, SIRT1 represents a promising therapeutic target in MASLD and related inflammatory liver diseases