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The Ni-NTA agarose was stripped with EDTA and recharged with iron as described 64

Three previously unknown reactions included the transsulfuration reaction to synthesize -Glu-Ser-Gly, the acetylation reaction to synthesize S-acetylglutathione, and the oxidation reaction to synthesize -Glu-3-sulfamoyl-Ala-Gly

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The CAT/SOD1 ratio also appears to be higher, although the difference is not significant, in patient cells compared with controls (Fig

( I and J ) Western blot analysis of FoxO1 and its quantification in the livers of ND- or HFD-fed LT and Fpn -LKO mice ( n = 68 per group)
