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The supernatant (storage buffer) was decanted, and the microspheres were resuspended in 50 l of 0.1 M MES [2-(N-morpholino) ethanesulfonic acid], pH 4.5

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Such was the situation of the two armies, when the attack of the Americans under Wayne and Howe upon Stony Point and Verplanck's Point was planned and executed by order of Washington 1779 On the morning of the 15th of July, all the Massachusetts light infantry were marched to the quarters of Wayne at Sandy Beach, fourteen miles from Stony Point

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