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Blood 98:764a, 2001

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Fragment 1 was cloned into Bgl II and Spe1 arms of the doxycycline inducible vector pDM310 [27] to generate a plasmid containing the linear sequence of Fragment 1 (pDM310-Frag1)

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After slices were dehydrated in progressively stronger alcohols, cleaned in xylene, and mounted, hematoxylin was used as a counterstain ( Measurement of the percentage area of a positive SIRT -1 immunoreaction in hepatic tissue Images were taken using a digital camera (Toucan type BX53, Japan) linked to a computer and a light microscope (Olympus model BX53, Tokyo, Japan)

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